| Potassium dihydrogen orthophosphate (KH2PO4) | 0.1 g |
| Ammonium nitrate (NH4NO3) | 0.1 g |
| Magnesium sulfate heptahydrate (MgSO4 7H2O) | 0.025 g |
| Yeast extract | 0.05 g |
| Distilled water to a total volume of | 100 ml |
| A nontoxic wetting agent such as sodium dioctyl sulfosuccinate or sodium lauryl sulfate | 0.005 g |
| Glycerol | 10.0 g |
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